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2](https:\u002F\u002Fp3-sign.toutiaoimg.com\u002Ftos-cn-i-tjoges91tu\u002F25f7d4a0f3d73614923e88cc6e5a50c2~tplv-tt-origin-web:gif.jpeg?_iz=58558&from=article.pc_detail&lk3s=953192f4&x-expires=1790208603&x-signature=ebJdUCVh5tB%2BOplz7JRPQfuCffg%3D)![Image 3](https:\u002F\u002Fp3-sign.toutiaoimg.com\u002Ftos-cn-i-tjoges91tu\u002F5bb4b4b75386d89a4038ffadcf0ee288~tplv-tt-origin-web:gif.jpeg?_iz=58558&from=article.pc_detail&lk3s=953192f4&x-expires=1790208603&x-signature=Cz%2F1kkBOtL2ImIp0w7eEC2RGKJY%3D)\n9月10日上午，全体参会代表赴农业农村部长丰生物育种科研试验基地开展田间观摩调研。本次活动地点地处江淮分水岭，拥有典型过渡性气候特征，可高度复刻长江中下游区域大田真实生产场景，能够为生物育种转化体材料开展南方生态区适应性“压力测试”提供理想试验条件。活动重点开展农作物转化体材料田间性状比对，直观呈现各类转化体材料的生长状态与综合农艺性状，以真实田间表现核验转化体材料应用价值，精准对接科研创新成果与农业生产实际需求。本次展示涵盖玉米、大豆、水稻三大作物，共计44份优质转化体材料。各类转化体材料长势均衡、性状优良、稳定性强。参会代表深入试验田块，近距离观测参试材料目标性状、田间表现，对比研判不同育转化体材料的综合优势、田间适配性及规模化推广潜力，为后续优良品种筛选、育种技术迭代升级、产业化推广应用筑牢实践根基。\n\n本次活动汇聚业内领导与专家，通过致辞赋能、前沿研讨，为生物育种产业创新发展明晰路径、凝聚科创力量。安徽省农业科学院党组成员、副院长李成在致辞中全面介绍了安徽省农科院在生物育种领域的科研积累、核心创新成果，以及助力种业振兴、保障粮食安全的工作成效。他表示，将以本次活动为契机，深化科企协同合作，畅通育种成果落地转化渠道，全力助推生物育种产业高质量发展。农业农村部科技发展中心纪委书记孙俊立指出，本次系列活动紧扣生物育种产业化痛点难点，兼具试验验证、成果展示、交流协作等多元价值，能够助力构建种业多方协同创新生态，为产业规范化、高质量发展理清发展思路。中国农业科学院科技管理局局长、国家玉米产业技术体系首席科学家李新海研究员，围绕农业转基因技术开展深度分享；中国作物学会大豆专业委员会主任委员、中国农业科学院作物科学研究所邱丽娟研究员，聚焦大豆生物育种产业化进行专项解读。\n\n![Image 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[举报](https:\u002F\u002Fwww.163.com\u002Fspecial\u002F0077jt\u002Ftipoff.html?title=Nature%20Plants%20%7C%20%E4%B8%AD%E5%B1%B1%E5%A4%A7%E5%AD%A6%E6%9D%8E%E5%89%91%E5%B3%B0%E5%9B%A2%E9%98%9FCRISPRa%E7%AD%9B%E9%80%89%E9%89%B4%E5%AE%9A%E6%B0%B4%E7%A8%BB%E5%B9%BF%E8%B0%B1%E6%8A%97%E7%97%85%E5%9F%BA%E5%9B%A0OsTV1)\n\n[](https:\u002F\u002Fwww.163.com\u002Fdy\u002Farticle\u002FL6ABK0UU0556K24E.html#post_comment_area \"快速发贴\")[0](https:\u002F\u002Fcomment.tie.163.com\u002FL6ABK0UU0556K24E.html \"点击查看跟贴\")\n\n分享至\n*   [](javascript:;)\n*   [](javascript:;)\n*   [](javascript:;)\n\n[](javascript:; \"关闭\")\n\n![Image 11: Scan me!](blob:http:\u002F\u002Flocalhost\u002Fb538c2cc233069cc0dc5d89cf993992d)\n\n用微信扫码二维码\n\n分享至好友和朋友圈\n\n植物基因功能研究长期依赖功能缺失筛选，然而植物基因组中存在大量功能冗余基因，单纯敲除单个基因往往不产生可见表型，并且某些关键基因的缺失会导致植株死亡，这些因素共同限制了传统筛选策略的效力和应用范围。2026年9月7日，中山大学生命科学学院**李剑峰**教授团队与浙江省农业科学院**寇艳君**研究员团队合作在Nature Plants期刊发表了题为“Cell-based CRISPRa screen identifies a papillae-specific receptor kinase mediating broad-spectrum fungal resistance in rice”的研究论文。该研究建立了一套基于水稻原生质体的dCas9-TV介导CRISPR激活遗传筛选平台，并利用这一平台从超过1300个水稻受体激酶和受体样胞质激酶基因中筛选到了广谱真菌抗性的正调控因子OsTV1。\n\n![Image 12](https:\u002F\u002Fnimg.ws.126.net\u002F?url=http%3A%2F%2Fdingyue.ws.126.net%2F2026%2F0908%2Fc8f4e992j00tl15e7001ud200p6009vg00p6009v.jpg&thumbnail=660x2147483647&quality=80&type=jpg)\n\n研究团队首先开发了一套适用于植物原生质体的高通量CRISPRa筛选流程。他们将经过改造的INTACT核标记系统置于稻瘟菌诱导型启动子OsChit6pro的控制之下，并与dCas9-TV和靶向近千个受体激酶基因启动子的混合gRNA文库共同转染水稻原生质体。当特定gRNA成功激活某个抗病正调控基因后，细胞内便会启动INTACT报告系统的表达，生物素标记的核膜蛋白随即能够通过链霉亲和素磁珠从大量细胞中分离纯化出来。研究人员进一步通过扩增子深度测序分析这些阳性细胞核中富集的gRNA序列，最终鉴定出了三个显著富集的候选基因。他们随后聚焦于其中一个编码LRR型受体激酶的未知功能基因并将其命名为OsTV1。\n\n为了验证OsTV1的生物学功能，研究团队分别构建了OsTV1过表达和CRISPR敲除的水稻遗传材料。表型分析结果显示，在正常生长条件下过表达植株和敲除植株的株高、分蘖数、结实率和籽粒千粒重等农艺性状均与野生型ZH11无显著差异，表明OsTV1的遗传操作不会对水稻正常生长发育产生明显不良影响。然而在接种稻瘟菌后，OsTV1过表达植株叶片上的病斑面积较野生型显著缩小，颈部稻瘟病发病等级明显降低，而OsTV1敲除植株则表现出更加严重的感病症状。这种抗病表型在田间自然发病条件下也得到了验证，并且过表达植株对水稻恶苗病和纹枯病同样表现出显著增强的抗性。\n\n![Image 13](https:\u002F\u002Fnimg.ws.126.net\u002F?url=http%3A%2F%2Fdingyue.ws.126.net%2F2026%2F0908%2Fd6e8fc58j00tl15e800khd200rr00uug00rr00uu.jpg&thumbnail=660x2147483647&quality=80&type=jpg)\n\n在机制研究方面，研究团队发现OsTV1特异性地在水稻叶片表皮乳突结构中表达，该结构已知是硅元素富集的部位。他们测定不同基因型材料叶片中的硅含量后发现，OsTV1过表达植株叶片中的硅含量较野生型显著升高，而敲除植株则显著降低。通过纳米压痕技术测量叶片力学性能，过表达植株的杨氏模量显著高于野生型，表明叶片刚性增强，敲除植株则相反。他们还通过免疫共沉淀实验证实OsTV1与硅外排转运蛋白OsSIET4存在蛋白质相互作用。转录组分析结果进一步显示，OsTV1过表达植株中木质素合成、植保素代谢和茉莉酸信号通路相关的防御基因表现出更高的基础表达水平和更强的诱导表达趋势。这些物理屏障强化和生化防御响应共同构成了OsTV1介导的广谱真菌抗性机制。\n\nREADING\n\nBioPeers\n\n特别声明：以上内容(如有图片或视频亦包括在内)为自媒体平台“网易号”用户上传并发布，本平台仅提供信息存储服务。\n\nNotice: The content above (including the pictures and videos if any) is uploaded and posted by a user of NetEase Hao, which is a social media platform and only provides information storage services.\n\n[![Image 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